Mitosis in mammalian cells relies on a microtubule structure known as the mitotic spindle, which is required to properly separate the parent cell’s genetic material. The mitotic spindle emanates from two centrosomes-microtubule organizing centers that play a major role in the formation and dynamics of the spindle. Treatment with the drug centrinone prevents the duplication of centrioles, a key component of the centrosome, and produces cells that cannot supply the two centrosomes normally required for mitosis. In this project, we quantify the effect of centrinone on both HeLa and LLCPK1 cells. First, we show that 48 hours is the optimal treatment time for producing a population with a high proportion of single-centrosome cells. Using this treatment duration, we then explore the visual differences in mitotic spindle formation and chromosome separation between cells with zero, one, and two centrosomes using live cell fluorescence microscopy. The spindles of single- centrosome cells were observed to be asymmetrical and prone to delays in division. Our objective is to quantify these differences in spindle shape and the duration of spindle formation in cells with normal spindle poles and cells lacking one or both centrosomes in order to further understand the role of the centrosome in mitosis. Mitochondria play an important role in the life of all eukaryotic organisms, including plants. The majority of the Geraniaceae are herbs, their leaves are usually lobed, opposites or alternate and the flowers are symmetrical. In this experiment, DNA was extracted from Pelargonium desertorum and sequenced. The sequencing was done by using the NanoPore MinION, which is suitable for sequencing long strands of DNA. Another advantage is that it doesn’t take a lot of time to get the sequence. The expected results of the project include the mitochondria genome sequence of Pelargonium desertorum. The challenge and advantages of sequencing plant mitochondrial genome will be discussed.
Accelerations of the head or pelvis can demonstrate in-phase or out-of-phase relationships. It is thought that in-phase movement is more stable, and this relationship is assessed with a harmonic ratio analysis. These ratios were determined from in-phase and out-of-phase accelerations within a subject’s stride rate where even harmonics are in-phase and odd harmonics are out-of-phase accelerations. A larger ratio is thought to correspond to a more stable gait pattern because more head. Pelvic movements are synchronized with the person’s stride. We hypothesized that walking asymmetrically will reduce the harmonic ratio. That reductions in asymmetry will coincide with increasing harmonic ratios. The results are expected to show alterations in the movement kinematics of the participants diagnosed with PFPS that will be exacerbated by pain onset during the run. The discovery of ShTPS2 is uniquely interesting due to its promiscuity. The rarity of characterized bifunctional sesterterpene synthases - terpene synthases with a chimeric prenyltransferase domain. 1) red blood cells to survive in the absence of any pore-forming toxin, as to minimize background noise; 2) render MMP2 active in order to cleave the inhibited pore-forming toxin; 3) render the wild type and cleaved mutant toxin active. My name is Amanda Lunn, and I’m a Graphic Design major from Salem State University. Art has always been a passion for me since I could hold a pencil, and Graphic Design is my way to harness my creativity into a career. One of my strongest interests is illustrating, so I like to portray that skill into aspects of my projects. I enjoy making my own graphical elements like iconography, lettings, and background when time constraints and the project calls for it. Ashley Jones is a Senior-year student currently pursuing a Bachelor of Arts degree in Graphic Design with a minor in Business Administration. Her portfolio presentation includes numerous piec
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